primary antibodies against ciap1  (R&D Systems)

Journal: Bioscience Reports

Article Title: cIAP1 promotes proliferation and migration and prevents apoptosis in gallbladder cancer in vitro

doi: 10.1042/BSR20182266

Figure Lengend Snippet: ( A ) Representative glance of expression of cIAP1 in GBC tissues at 200×. ( B ) Representative glance of expression of cIAP1 in non-tumor tissues at 200×. ( C ) Magnified picture of hotspot from corresponding area in GBC tissue at 400×. ( D ) Magnified picture of hotspot from corresponding area in non-tumor tissues GBC tissue at 400×.

Article Snippet: Primary antibodies against cIAP1 (1:400, CST) were incubated with the sections overnight at 4°C.

Techniques: Expressing

Journal: Bioscience Reports

Article Title: cIAP1 promotes proliferation and migration and prevents apoptosis in gallbladder cancer in vitro

doi: 10.1042/BSR20182266

Figure Lengend Snippet: The expression of cIAP1 measured by MOD in GBC tissues and matched non-tumor tissues

Article Snippet: Primary antibodies against cIAP1 (1:400, CST) were incubated with the sections overnight at 4°C.

Techniques: Expressing

Journal: Bioscience Reports

Article Title: cIAP1 promotes proliferation and migration and prevents apoptosis in gallbladder cancer in vitro

doi: 10.1042/BSR20182266

Figure Lengend Snippet: ( A , B ) LV-shcIAP1 group have Caspase-8 cleaved and activated more readily and severely than LV-NC in periodical Western blot in 6 h. ( C , D ) Another arrangement of group of various combinations of stimulation were treated with two GBC cells with indicated dose and time. The group treating TNF-α/cycloheximide showed obvious decrease in cIAP1 and increase in cleaved PARP, cleaved Caspase-3, and cleaved Caspase-8. Proteins with proximate molecular weight were performed by separated gels in the same electrophoresis with loading control of GAPDH in triplicate.

Article Snippet: Primary antibodies against cIAP1 (1:400, CST) were incubated with the sections overnight at 4°C.

Techniques: Western Blot, Molecular Weight, Electrophoresis

Journal: Bioscience Reports

Article Title: cIAP1 promotes proliferation and migration and prevents apoptosis in gallbladder cancer in vitro

doi: 10.1042/BSR20182266

Figure Lengend Snippet: ( A , B ) The immunoprecipitation analysis prior co-treatment of TNF-α and cycloheximide showed Caspase-8 with RIP1 but knockdown of cIAP1 failed to enhance such interaction with reference to Input ones. ( C , D ) There was less translocation of NF-κB subunit P65 from plasma to nuclear in LV-shcIAP1 group under the stimulation of TNF-α for 6 h in both cell lines. ( E – G ) The expressions of c-FLIP in both transcriptional and translational levels were reduced in LV-shcIAP1 group in the stimulation of TNF-α (* P < 0.05, ** P < 0.01). Similar results were assured at least triplicated.

Article Snippet: Primary antibodies against cIAP1 (1:400, CST) were incubated with the sections overnight at 4°C.

Techniques: Immunoprecipitation, Translocation Assay

Journal: Blood Advances

Article Title: The SMAC mimetic LCL-161 displays antitumor activity in preclinical models of rituximab-resistant B-cell lymphoma

doi: 10.1182/bloodadvances.2018018168

Figure Lengend Snippet: The expression of survivin and livin is upregulated in RRCLs. (A) Western blot analysis indicated that the IAPs livin and survivin were upregulated in the RRCLs (Raji 2R, Raji 4RH, and RL 4RH). The expression of cIAP1 and XIAP was moderately increased, whereas no change was observed in the level of cIAP2 between RRCLs and sensitive parent cell lines. (B) Quantitative PCR results demonstrate an increase in expression of the Birc5 and Birc7 genes, which encode the survivin and livin proteins, respectively, in the RRCLs Raji 4RH and RL 4RH compared with rituximab-sensitive control cell lines.

Article Snippet: Primary western blot antibodies against human IAPs (XIAP, livin, cIAP1/2, and Bruce) were purchased from Cell Signaling Technology (Danvers, MA), along with antibodies against β-actin and poly (ADP-ribose) polymerase (PARP).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Control

Journal: Blood Advances

Article Title: The SMAC mimetic LCL-161 displays antitumor activity in preclinical models of rituximab-resistant B-cell lymphoma

doi: 10.1182/bloodadvances.2018018168

Figure Lengend Snippet: LCL-161 decreases the expression of several IAPs and induces apoptosis. (A-B) The rituximab-sensitive lymphoma cell line Raji/RL and the rituximab-resistant clone Raji 4RH/RL 4RH were exposed to escalating doses of LCL-161 (0-40 μM, as indicated) for 24 hours prior to protein extraction. LCL-161 decreased the expression of cIAP1, cIAP2, and livin in a dose-dependent manner at 24 hours postexposure. LCL-161 induced PARP cleavage at concentrations of 20 and 40 μM in the resistant Raji 4RH cell line. In RL cells, LCL-161 decreased the expression cIAP1, survivin, and livin in a dose-dependent manner at 24 hours postexposure. LCL-161 induced PARP cleavage at concentrations of 20 and 40 μM in the RL cell line, but not in the RL 4RH cell line. (C) LCL-161 produces a dose-dependent increase in caspase 3/7 activity in the germinal center B-cell DLBCL cell line Karpas 422 at 48 hours.

Article Snippet: Primary western blot antibodies against human IAPs (XIAP, livin, cIAP1/2, and Bruce) were purchased from Cell Signaling Technology (Danvers, MA), along with antibodies against β-actin and poly (ADP-ribose) polymerase (PARP).

Techniques: Expressing, Protein Extraction, Activity Assay